59.7 Homocysteine

59.7.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

Prolactin
Chisq Df Pr(>Chisq)
(Intercept) 145.0597858 1 <0.0001 ***
Homocysteine 1.4645859 1 0.2 ns
GDM_new 2.3423556 1 0.1 ns
Study 0.0346867 1 0.9 ns
GA_samp 139.7575423 1 <0.0001 ***
Homocysteine:GDM_new 1.7444634 1 0.2 ns
GDM_new:Study 3.8352749 1 0.05 ns
Homocysteine:Study 0.0118213 1 0.9 ns
Homocysteine:GDM_new:Study 2.7686048 1 0.10 ns
HPL
Chisq Df Pr(>Chisq)
(Intercept) 36.4449860 1 <0.0001 ***
Homocysteine 0.6556876 1 0.4 ns
GDM_new 0.1042487 1 0.7 ns
Study 2.8477539 1 0.09 ns
GA_samp 1872.6604971 1 <0.0001 ***
Homocysteine:GDM_new 0.4873806 1 0.5 ns
GDM_new:Study 0.5441908 1 0.5 ns
Homocysteine:Study 0.0811476 1 0.8 ns
Homocysteine:GDM_new:Study 0.1343363 1 0.7 ns
GH2
Chisq Df Pr(>Chisq)
(Intercept) 235.4814378 1 <0.0001 ***
Homocysteine 1.2749445 1 0.3 ns
GDM_new 0.0108539 1 0.9 ns
Study 1.0983585 1 0.3 ns
GA_samp 857.3642845 1 <0.0001 ***
Homocysteine:GDM_new 0.1125577 1 0.7 ns
GDM_new:Study 0.5351343 1 0.5 ns
Homocysteine:Study 0.9971124 1 0.3 ns
Homocysteine:GDM_new:Study 0.0389948 1 0.8 ns
Insulin
model term df1 df2 F.ratio Chisq p.value
1 Homocysteine 1 Inf 19.971 19.971 <0.0001 ***
5 GDM_new 1 Inf 32.054 32.054 <0.0001 ***
7 Study 1 Inf 11.698 11.698 0.0006 ***
8 GA_samp 1 Inf 0.002 0.002 1.0 ns
2 Homocysteine:GDM_new 1 Inf 15.897 15.897 <0.0001 ***
4 Homocysteine:Study 1 Inf 6.473 6.473 0.01 *
6 GDM_new:Study 1 Inf 6.743 6.743 0.009 **
3 Homocysteine:GDM_new:Study 1 Inf 6.386 6.386 0.01 *
HOMA.IR
model term df1 df2 F.ratio Chisq p.value
1 Homocysteine 1 Inf 21.949 21.949 <0.0001 ***
5 GDM_new 1 Inf 35.671 35.671 <0.0001 ***
7 Study 1 Inf 17.025 17.025 <0.0001 ***
8 GA_samp 1 Inf 0.034 0.034 0.9 ns
2 Homocysteine:GDM_new 1 Inf 18.293 18.293 <0.0001 ***
4 Homocysteine:Study 1 Inf 8.289 8.289 0.004 **
6 GDM_new:Study 1 Inf 8.818 8.818 0.003 **
3 Homocysteine:GDM_new:Study 1 Inf 8.172 8.172 0.004 **
GLUC3_mmol.L
model term df1 df2 F.ratio Chisq p.value
1 Homocysteine 1 Inf 8.564 8.564 0.003 **
5 GDM_new 1 Inf 9.165 9.165 0.002 **
7 Study 1 Inf 13.971 13.971 0.0002 ***
8 GA_samp 1 Inf 0.961 0.961 0.3 ns
2 Homocysteine:GDM_new 1 Inf 7.553 7.553 0.006 **
4 Homocysteine:Study 1 Inf 5.823 5.823 0.02 *
6 GDM_new:Study 1 Inf 7.209 7.209 0.007 **
3 Homocysteine:GDM_new:Study 1 Inf 7.141 7.141 0.008 **

59.7.2 Model summary

Ratio of Geometric means (95% CI)

Prolactin
Var Effect P
(Intercept) 2.79 (2.33, 3.25) <0.0001
Homocysteine -0.02 (-0.07, 0.03) 0.37
GDM_newGDM -0.01 (-0.44, 0.42) 0.96
StudySTOP 0.03 (-0.28, 0.35) 0.83
GA_samp 0.14 (0.12, 0.17) <0.0001
Homocysteine:GDM_newGDM -0.03 (-0.12, 0.06) 0.56
GDM_newGDM:StudySTOP 0.19 (-0.05, 0.43) 0.12
Homocysteine:StudySTOP -0.01 (-0.07, 0.05) 0.74
HPL
Var Effect P
(Intercept) 0.74 (0.50, 0.98) <0.0001
Homocysteine -0.01 (-0.04, 0.02) 0.45
GDM_newGDM 0.17 (-0.06, 0.40) 0.14
StudySTOP 0.16 (-0.01, 0.33) 0.07
GA_samp 0.29 (0.28, 0.30) <0.0001
Homocysteine:GDM_newGDM -0.07 (-0.11, -0.02) 0.010
GDM_newGDM:StudySTOP 0.11 (-0.01, 0.24) 0.07
Homocysteine:StudySTOP -0.01 (-0.04, 0.03) 0.69
GH2
Var Effect P
(Intercept) -2.51 (-2.83, -2.19) <0.0001
Homocysteine -0.02 (-0.06, 0.02) 0.27
GDM_newGDM 0.03 (-0.28, 0.34) 0.85
StudySTOP -0.12 (-0.35, 0.11) 0.30
GA_samp 0.26 (0.24, 0.28) <0.0001
Homocysteine:GDM_newGDM -0.05 (-0.11, 0.02) 0.16
GDM_newGDM:StudySTOP 0.23 (0.06, 0.40) 0.009
Homocysteine:StudySTOP 0.02 (-0.02, 0.07) 0.33
Insulin
Var Effect P
(Intercept) 2.74 (2.12, 3.37) <0.0001
Homocysteine -0.02 (-0.10, 0.06) 0.60
GDM_newGDM 3.00 (1.82, 4.18) <0.0001
StudySTOP -0.31 (-0.78, 0.16) 0.19
GA_samp 0.00 (-0.03, 0.03) 0.97
Homocysteine:GDM_newGDM -0.53 (-0.81, -0.26) 0.0002
GDM_newGDM:StudySTOP -1.89 (-3.31, -0.46) 0.009
Homocysteine:StudySTOP 0.00 (-0.10, 0.10) 0.98
Homocysteine:GDM_newGDM:StudySTOP 0.41 (0.09, 0.73) 0.01
HOMA.IR
Var Effect P
(Intercept) 1.37 (0.71, 2.03) <0.0001
Homocysteine -0.02 (-0.10, 0.06) 0.65
GDM_newGDM 3.52 (2.23, 4.82) <0.0001
StudySTOP -0.47 (-0.97, 0.04) 0.07
GA_samp -0.00 (-0.04, 0.03) 0.85
Homocysteine:GDM_newGDM -0.64 (-0.94, -0.33) <0.0001
GDM_newGDM:StudySTOP -2.34 (-3.89, -0.80) 0.003
Homocysteine:StudySTOP 0.00 (-0.10, 0.11) 0.97
Homocysteine:GDM_newGDM:StudySTOP 0.51 (0.16, 0.86) 0.004
GLUC3_mmol.L
Var Effect P
(Intercept) 1.74 (1.60, 1.87) <0.0001
Homocysteine 0.00 (-0.01, 0.02) 0.90
GDM_newGDM 0.68 (0.23, 1.12) 0.003
StudySTOP -0.12 (-0.22, -0.01) 0.03
GA_samp -0.00 (-0.01, 0.00) 0.33
Homocysteine:GDM_newGDM -0.14 (-0.24, -0.04) 0.005
GDM_newGDM:StudySTOP -0.64 (-1.10, -0.17) 0.007
Homocysteine:StudySTOP -0.01 (-0.03, 0.01) 0.56
Homocysteine:GDM_newGDM:StudySTOP 0.14 (0.04, 0.24) 0.008

59.7.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

Results are averaged over the levels of Homocysteine concentration (μM) and may be misleading when interaction term is involved.

Prolactin
Study GDM_new response SE df lower.CL upper.CL
SCOPE Uncomplicated 94.96759 7.759243 107.35549 80.76736 111.6645
STOP Uncomplicated 93.13168 6.970409 76.08526 80.23430 108.1023
SCOPE GDM 82.02212 11.008890 571.00076 63.01461 106.7630
STOP GDM 97.22880 8.592851 142.95040 81.64422 115.7882
HPL
Study GDM_new response SE df lower.CL upper.CL
SCOPE Uncomplicated 86.42107 2.601200 205.8178 81.44186 91.70471
STOP Uncomplicated 98.01198 2.488169 106.3098 93.20119 103.07109
SCOPE GDM 74.00851 4.536267 804.1712 65.61900 83.47063
STOP GDM 93.98161 3.363938 321.4001 87.59114 100.83832
GH2
Study GDM_new response SE df lower.CL upper.CL
SCOPE Uncomplicated 2.223348 0.0765585 475.7601 2.077890 2.378989
STOP Uncomplicated 2.212053 0.0579810 206.6213 2.100646 2.329369
SCOPE GDM 1.802244 0.1524281 1191.9659 1.526681 2.127544
STOP GDM 2.257613 0.0944288 501.2926 2.079507 2.450975
Insulin
Study GDM_new response SE df asymp.LCL asymp.UCL
SCOPE Uncomplicated 14.11386 0.9262269 Inf 12.410387 16.05115
STOP Uncomplicated 10.38412 0.4296079 Inf 9.575338 11.26122
SCOPE GDM 19.96015 3.6307532 Inf 13.974303 28.51001
STOP GDM 17.43694 1.3414818 Inf 14.996317 20.27478
HOMA.IR
Study GDM_new response SE df asymp.LCL asymp.UCL
SCOPE Uncomplicated 3.426268 0.2408458 Inf 2.985294 3.932382
STOP Uncomplicated 2.163895 0.0941183 Inf 1.987070 2.356454
SCOPE GDM 4.807918 0.9882138 Inf 3.213676 7.193032
STOP GDM 3.750223 0.3083980 Inf 3.191970 4.406109
GLUC3_mmol.L
Study GDM_new response SE df asymp.LCL asymp.UCL
SCOPE Uncomplicated 5.447121 0.0654643 Inf 5.320313 5.576952
STOP Uncomplicated 4.701641 0.0394811 Inf 4.624892 4.779663
SCOPE GDM 5.340856 0.2627192 Inf 4.849979 5.881416
STOP GDM 4.850957 0.0626863 Inf 4.729637 4.975390

59.7.4 Comparisons

59.7.4.1 Within Groups

Effect for every 1 unit increase in Homocysteine concentration (μM).
Hormone Contrast Study GDM_new Ratio of Geometric means (95% CI) P-value
Prolactin Homocysteine5.5 / Homocysteine4.5 SCOPE Uncomplicated 0.978 (0.930, 1.027) 0.4
Prolactin Homocysteine5.5 / Homocysteine4.5 STOP Uncomplicated 0.967 (0.927, 1.009) 0.1
Prolactin Homocysteine5.5 / Homocysteine4.5 SCOPE GDM 0.952 (0.860, 1.053) 0.3
Prolactin Homocysteine5.5 / Homocysteine4.5 STOP GDM 0.941 (0.865, 1.024) 0.2
HPL Homocysteine5.5 / Homocysteine4.5 SCOPE Uncomplicated 0.990 (0.964, 1.017) 0.5
HPL Homocysteine5.5 / Homocysteine4.5 STOP Uncomplicated 0.983 (0.961, 1.006) 0.1
HPL Homocysteine5.5 / Homocysteine4.5 SCOPE GDM 0.927 (0.878, 0.979) 0.006
HPL Homocysteine5.5 / Homocysteine4.5 STOP GDM 0.921 (0.879, 0.964) 0.0004
GH2 Homocysteine5.5 / Homocysteine4.5 SCOPE Uncomplicated 0.980 (0.946, 1.016) 0.3
GH2 Homocysteine5.5 / Homocysteine4.5 STOP Uncomplicated 1.003 (0.973, 1.035) 0.8
GH2 Homocysteine5.5 / Homocysteine4.5 SCOPE GDM 0.934 (0.868, 1.006) 0.07
GH2 Homocysteine5.5 / Homocysteine4.5 STOP GDM 0.956 (0.899, 1.017) 0.2
Insulin Homocysteine5.5 / Homocysteine4.5 SCOPE Uncomplicated 0.979 (0.905, 1.059) 0.6
Insulin Homocysteine5.5 / Homocysteine4.5 STOP Uncomplicated 0.980 (0.926, 1.038) 0.5
Insulin Homocysteine5.5 / Homocysteine4.5 SCOPE GDM 0.576 (0.442, 0.750) <0.0001
Insulin Homocysteine5.5 / Homocysteine4.5 STOP GDM 0.870 (0.747, 1.014) 0.07
HOMA.IR Homocysteine5.5 / Homocysteine4.5 SCOPE Uncomplicated 0.980 (0.901, 1.067) 0.6
HOMA.IR Homocysteine5.5 / Homocysteine4.5 STOP Uncomplicated 0.982 (0.924, 1.044) 0.6
HOMA.IR Homocysteine5.5 / Homocysteine4.5 SCOPE GDM 0.518 (0.387, 0.695) <0.0001
HOMA.IR Homocysteine5.5 / Homocysteine4.5 STOP GDM 0.865 (0.736, 1.017) 0.08
GLUC3_mmol.L Homocysteine5.5 / Homocysteine4.5 SCOPE Uncomplicated 1.001 (0.987, 1.015) 0.9
GLUC3_mmol.L Homocysteine5.5 / Homocysteine4.5 STOP Uncomplicated 0.995 (0.980, 1.010) 0.5
GLUC3_mmol.L Homocysteine5.5 / Homocysteine4.5 SCOPE GDM 0.870 (0.790, 0.958) 0.005
GLUC3_mmol.L Homocysteine5.5 / Homocysteine4.5 STOP GDM 0.993 (0.971, 1.016) 0.5

59.7.4.2 Between Groups

Below are comparisons of the differences in the change in Homocysteine concentration (μM) between groups. I.e. whether the change in Homocysteine concentration (μM) are different between groups.

Prolactin
contrast ratio SE df lower.CL upper.CL p.value
SCOPE/SCOPE (Overall) 0.9787372 0.0640855 1417.025 0.8607632 1.112880 0.743 ns
STOP/SCOPE (Uncomplicated) 0.9893115 0.0323889 1417.025 0.9277733 1.054931 0.743 ns
STOP/SCOPE (GDM) 0.9893115 0.0323889 1417.025 0.9277733 1.054931 0.743 ns
GDM/Uncomplicated (SCOPE) 0.9732322 0.0452715 1412.965 0.8883569 1.066217 0.560 ns
GDM/Uncomplicated (STOP) 0.9732322 0.0452715 1412.965 0.8883569 1.066217 0.560 ns
HPL
contrast ratio SE df lower.CL upper.CL p.value
SCOPE/SCOPE (Overall) 0.9861437 0.0348326 1400.365 0.9201276 1.0568962 0.693 ns
STOP/SCOPE (Uncomplicated) 0.9930477 0.0175382 1400.365 0.9592328 1.0280546 0.693 ns
STOP/SCOPE (GDM) 0.9930477 0.0175382 1400.365 0.9592328 1.0280546 0.693 ns
GDM/Uncomplicated (SCOPE) 0.9366159 0.0236704 1397.986 0.8913147 0.9842196 0.010 **
GDM/Uncomplicated (STOP) 0.9366159 0.0236704 1397.986 0.8913147 0.9842196 0.010 **
GH2
contrast ratio SE df lower.CL upper.CL p.value
SCOPE/SCOPE (Overall) 1.0475947 0.0496954 1468.841 0.9545111 1.149756 0.327 ns
STOP/SCOPE (Uncomplicated) 1.0235207 0.0242767 1468.841 0.9769909 1.072267 0.327 ns
STOP/SCOPE (GDM) 1.0235207 0.0242767 1468.841 0.9769909 1.072267 0.327 ns
GDM/Uncomplicated (SCOPE) 0.9532964 0.0323553 1427.051 0.8918941 1.018926 0.159 ns
GDM/Uncomplicated (STOP) 0.9532964 0.0323553 1427.051 0.8918941 1.018926 0.159 ns
Insulin
contrast ratio SE df asymp.LCL asymp.UCL p.value
SCOPE/SCOPE (Overall) 1.5134372 0.2464932 Inf 1.0998398 2.082569 0.011 *
STOP/SCOPE (Uncomplicated) 1.0011112 0.0496046 Inf 0.9084598 1.103212 0.982 ns
STOP/SCOPE (GDM) 1.5117573 0.2347271 Inf 1.1151106 2.049492 0.008 **
GDM/Uncomplicated (SCOPE) 0.5879472 0.0826295 Inf 0.4463867 0.774400 0.0002 ***
GDM/Uncomplicated (STOP) 0.8878469 0.0734313 Inf 0.7549838 1.044091 0.150 ns
HOMA.IR
contrast ratio SE df asymp.LCL asymp.UCL p.value
SCOPE/SCOPE (Overall) 1.6721775 0.2986039 Inf 1.1783700 2.3729199 0.004 **
STOP/SCOPE (Uncomplicated) 1.0017903 0.0535375 Inf 0.9021672 1.1124143 0.973 ns
STOP/SCOPE (GDM) 1.6691892 0.2844275 Inf 1.1952591 2.3310367 0.003 **
GDM/Uncomplicated (SCOPE) 0.5288124 0.0822546 Inf 0.3898528 0.7173029 <0.0001 ***
GDM/Uncomplicated (STOP) 0.8811105 0.0772880 Inf 0.7419351 1.0463931 0.149 ns
GLUC3_mmol.L
contrast ratio SE df asymp.LCL asymp.UCL p.value
SCOPE/SCOPE (Overall) 1.1337832 0.0589925 Inf 1.0238604 1.2555076 0.016 *
STOP/SCOPE (Uncomplicated) 0.9938672 0.0106101 Inf 0.9732879 1.0148816 0.564 ns
STOP/SCOPE (GDM) 1.1407794 0.0578375 Inf 1.0328702 1.2599624 0.009 **
GDM/Uncomplicated (SCOPE) 0.8696156 0.0432179 Inf 0.7889047 0.9585839 0.005 **
GDM/Uncomplicated (STOP) 0.9981612 0.0136557 Inf 0.9717522 1.0252879 0.893 ns

59.7.5 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

59.7.6 Plots