59.7 Homocysteine
59.7.1 Global tests
Type III ANOVA on main and interaction effects, and LM model summaries.
Prolactin| Chisq | Df | Pr(>Chisq) | ||
|---|---|---|---|---|
| (Intercept) | 145.0597858 | 1 | <0.0001 | *** |
| Homocysteine | 1.4645859 | 1 | 0.2 | ns |
| GDM_new | 2.3423556 | 1 | 0.1 | ns |
| Study | 0.0346867 | 1 | 0.9 | ns |
| GA_samp | 139.7575423 | 1 | <0.0001 | *** |
| Homocysteine:GDM_new | 1.7444634 | 1 | 0.2 | ns |
| GDM_new:Study | 3.8352749 | 1 | 0.05 | ns |
| Homocysteine:Study | 0.0118213 | 1 | 0.9 | ns |
| Homocysteine:GDM_new:Study | 2.7686048 | 1 | 0.10 | ns |
| Chisq | Df | Pr(>Chisq) | ||
|---|---|---|---|---|
| (Intercept) | 36.4449860 | 1 | <0.0001 | *** |
| Homocysteine | 0.6556876 | 1 | 0.4 | ns |
| GDM_new | 0.1042487 | 1 | 0.7 | ns |
| Study | 2.8477539 | 1 | 0.09 | ns |
| GA_samp | 1872.6604971 | 1 | <0.0001 | *** |
| Homocysteine:GDM_new | 0.4873806 | 1 | 0.5 | ns |
| GDM_new:Study | 0.5441908 | 1 | 0.5 | ns |
| Homocysteine:Study | 0.0811476 | 1 | 0.8 | ns |
| Homocysteine:GDM_new:Study | 0.1343363 | 1 | 0.7 | ns |
| Chisq | Df | Pr(>Chisq) | ||
|---|---|---|---|---|
| (Intercept) | 235.4814378 | 1 | <0.0001 | *** |
| Homocysteine | 1.2749445 | 1 | 0.3 | ns |
| GDM_new | 0.0108539 | 1 | 0.9 | ns |
| Study | 1.0983585 | 1 | 0.3 | ns |
| GA_samp | 857.3642845 | 1 | <0.0001 | *** |
| Homocysteine:GDM_new | 0.1125577 | 1 | 0.7 | ns |
| GDM_new:Study | 0.5351343 | 1 | 0.5 | ns |
| Homocysteine:Study | 0.9971124 | 1 | 0.3 | ns |
| Homocysteine:GDM_new:Study | 0.0389948 | 1 | 0.8 | ns |
| model term | df1 | df2 | F.ratio | Chisq | p.value | ||
|---|---|---|---|---|---|---|---|
| 1 | Homocysteine | 1 | Inf | 19.971 | 19.971 | <0.0001 | *** |
| 5 | GDM_new | 1 | Inf | 32.054 | 32.054 | <0.0001 | *** |
| 7 | Study | 1 | Inf | 11.698 | 11.698 | 0.0006 | *** |
| 8 | GA_samp | 1 | Inf | 0.002 | 0.002 | 1.0 | ns |
| 2 | Homocysteine:GDM_new | 1 | Inf | 15.897 | 15.897 | <0.0001 | *** |
| 4 | Homocysteine:Study | 1 | Inf | 6.473 | 6.473 | 0.01 | * |
| 6 | GDM_new:Study | 1 | Inf | 6.743 | 6.743 | 0.009 | ** |
| 3 | Homocysteine:GDM_new:Study | 1 | Inf | 6.386 | 6.386 | 0.01 | * |
| model term | df1 | df2 | F.ratio | Chisq | p.value | ||
|---|---|---|---|---|---|---|---|
| 1 | Homocysteine | 1 | Inf | 21.949 | 21.949 | <0.0001 | *** |
| 5 | GDM_new | 1 | Inf | 35.671 | 35.671 | <0.0001 | *** |
| 7 | Study | 1 | Inf | 17.025 | 17.025 | <0.0001 | *** |
| 8 | GA_samp | 1 | Inf | 0.034 | 0.034 | 0.9 | ns |
| 2 | Homocysteine:GDM_new | 1 | Inf | 18.293 | 18.293 | <0.0001 | *** |
| 4 | Homocysteine:Study | 1 | Inf | 8.289 | 8.289 | 0.004 | ** |
| 6 | GDM_new:Study | 1 | Inf | 8.818 | 8.818 | 0.003 | ** |
| 3 | Homocysteine:GDM_new:Study | 1 | Inf | 8.172 | 8.172 | 0.004 | ** |
| model term | df1 | df2 | F.ratio | Chisq | p.value | ||
|---|---|---|---|---|---|---|---|
| 1 | Homocysteine | 1 | Inf | 8.564 | 8.564 | 0.003 | ** |
| 5 | GDM_new | 1 | Inf | 9.165 | 9.165 | 0.002 | ** |
| 7 | Study | 1 | Inf | 13.971 | 13.971 | 0.0002 | *** |
| 8 | GA_samp | 1 | Inf | 0.961 | 0.961 | 0.3 | ns |
| 2 | Homocysteine:GDM_new | 1 | Inf | 7.553 | 7.553 | 0.006 | ** |
| 4 | Homocysteine:Study | 1 | Inf | 5.823 | 5.823 | 0.02 | * |
| 6 | GDM_new:Study | 1 | Inf | 7.209 | 7.209 | 0.007 | ** |
| 3 | Homocysteine:GDM_new:Study | 1 | Inf | 7.141 | 7.141 | 0.008 | ** |
59.7.2 Model summary
Ratio of Geometric means (95% CI)
Prolactin| Var | Effect | P |
|---|---|---|
| (Intercept) | 2.79 (2.33, 3.25) | <0.0001 |
| Homocysteine | -0.02 (-0.07, 0.03) | 0.37 |
| GDM_newGDM | -0.01 (-0.44, 0.42) | 0.96 |
| StudySTOP | 0.03 (-0.28, 0.35) | 0.83 |
| GA_samp | 0.14 (0.12, 0.17) | <0.0001 |
| Homocysteine:GDM_newGDM | -0.03 (-0.12, 0.06) | 0.56 |
| GDM_newGDM:StudySTOP | 0.19 (-0.05, 0.43) | 0.12 |
| Homocysteine:StudySTOP | -0.01 (-0.07, 0.05) | 0.74 |
| Var | Effect | P |
|---|---|---|
| (Intercept) | 0.74 (0.50, 0.98) | <0.0001 |
| Homocysteine | -0.01 (-0.04, 0.02) | 0.45 |
| GDM_newGDM | 0.17 (-0.06, 0.40) | 0.14 |
| StudySTOP | 0.16 (-0.01, 0.33) | 0.07 |
| GA_samp | 0.29 (0.28, 0.30) | <0.0001 |
| Homocysteine:GDM_newGDM | -0.07 (-0.11, -0.02) | 0.010 |
| GDM_newGDM:StudySTOP | 0.11 (-0.01, 0.24) | 0.07 |
| Homocysteine:StudySTOP | -0.01 (-0.04, 0.03) | 0.69 |
| Var | Effect | P |
|---|---|---|
| (Intercept) | -2.51 (-2.83, -2.19) | <0.0001 |
| Homocysteine | -0.02 (-0.06, 0.02) | 0.27 |
| GDM_newGDM | 0.03 (-0.28, 0.34) | 0.85 |
| StudySTOP | -0.12 (-0.35, 0.11) | 0.30 |
| GA_samp | 0.26 (0.24, 0.28) | <0.0001 |
| Homocysteine:GDM_newGDM | -0.05 (-0.11, 0.02) | 0.16 |
| GDM_newGDM:StudySTOP | 0.23 (0.06, 0.40) | 0.009 |
| Homocysteine:StudySTOP | 0.02 (-0.02, 0.07) | 0.33 |
| Var | Effect | P |
|---|---|---|
| (Intercept) | 2.74 (2.12, 3.37) | <0.0001 |
| Homocysteine | -0.02 (-0.10, 0.06) | 0.60 |
| GDM_newGDM | 3.00 (1.82, 4.18) | <0.0001 |
| StudySTOP | -0.31 (-0.78, 0.16) | 0.19 |
| GA_samp | 0.00 (-0.03, 0.03) | 0.97 |
| Homocysteine:GDM_newGDM | -0.53 (-0.81, -0.26) | 0.0002 |
| GDM_newGDM:StudySTOP | -1.89 (-3.31, -0.46) | 0.009 |
| Homocysteine:StudySTOP | 0.00 (-0.10, 0.10) | 0.98 |
| Homocysteine:GDM_newGDM:StudySTOP | 0.41 (0.09, 0.73) | 0.01 |
| Var | Effect | P |
|---|---|---|
| (Intercept) | 1.37 (0.71, 2.03) | <0.0001 |
| Homocysteine | -0.02 (-0.10, 0.06) | 0.65 |
| GDM_newGDM | 3.52 (2.23, 4.82) | <0.0001 |
| StudySTOP | -0.47 (-0.97, 0.04) | 0.07 |
| GA_samp | -0.00 (-0.04, 0.03) | 0.85 |
| Homocysteine:GDM_newGDM | -0.64 (-0.94, -0.33) | <0.0001 |
| GDM_newGDM:StudySTOP | -2.34 (-3.89, -0.80) | 0.003 |
| Homocysteine:StudySTOP | 0.00 (-0.10, 0.11) | 0.97 |
| Homocysteine:GDM_newGDM:StudySTOP | 0.51 (0.16, 0.86) | 0.004 |
| Var | Effect | P |
|---|---|---|
| (Intercept) | 1.74 (1.60, 1.87) | <0.0001 |
| Homocysteine | 0.00 (-0.01, 0.02) | 0.90 |
| GDM_newGDM | 0.68 (0.23, 1.12) | 0.003 |
| StudySTOP | -0.12 (-0.22, -0.01) | 0.03 |
| GA_samp | -0.00 (-0.01, 0.00) | 0.33 |
| Homocysteine:GDM_newGDM | -0.14 (-0.24, -0.04) | 0.005 |
| GDM_newGDM:StudySTOP | -0.64 (-1.10, -0.17) | 0.007 |
| Homocysteine:StudySTOP | -0.01 (-0.03, 0.01) | 0.56 |
| Homocysteine:GDM_newGDM:StudySTOP | 0.14 (0.04, 0.24) | 0.008 |
59.7.3 Estimated means
Estimated marginal means (or adjusted means) adjusted for GA at sampling.
Results are averaged over the levels of Homocysteine concentration (μM) and may be misleading when interaction term is involved.
| Study | GDM_new | response | SE | df | lower.CL | upper.CL |
|---|---|---|---|---|---|---|
| SCOPE | Uncomplicated | 94.96759 | 7.759243 | 107.35549 | 80.76736 | 111.6645 |
| STOP | Uncomplicated | 93.13168 | 6.970409 | 76.08526 | 80.23430 | 108.1023 |
| SCOPE | GDM | 82.02212 | 11.008890 | 571.00076 | 63.01461 | 106.7630 |
| STOP | GDM | 97.22880 | 8.592851 | 142.95040 | 81.64422 | 115.7882 |
| Study | GDM_new | response | SE | df | lower.CL | upper.CL |
|---|---|---|---|---|---|---|
| SCOPE | Uncomplicated | 86.42107 | 2.601200 | 205.8178 | 81.44186 | 91.70471 |
| STOP | Uncomplicated | 98.01198 | 2.488169 | 106.3098 | 93.20119 | 103.07109 |
| SCOPE | GDM | 74.00851 | 4.536267 | 804.1712 | 65.61900 | 83.47063 |
| STOP | GDM | 93.98161 | 3.363938 | 321.4001 | 87.59114 | 100.83832 |
| Study | GDM_new | response | SE | df | lower.CL | upper.CL |
|---|---|---|---|---|---|---|
| SCOPE | Uncomplicated | 2.223348 | 0.0765585 | 475.7601 | 2.077890 | 2.378989 |
| STOP | Uncomplicated | 2.212053 | 0.0579810 | 206.6213 | 2.100646 | 2.329369 |
| SCOPE | GDM | 1.802244 | 0.1524281 | 1191.9659 | 1.526681 | 2.127544 |
| STOP | GDM | 2.257613 | 0.0944288 | 501.2926 | 2.079507 | 2.450975 |
| Study | GDM_new | response | SE | df | asymp.LCL | asymp.UCL |
|---|---|---|---|---|---|---|
| SCOPE | Uncomplicated | 14.11386 | 0.9262269 | Inf | 12.410387 | 16.05115 |
| STOP | Uncomplicated | 10.38412 | 0.4296079 | Inf | 9.575338 | 11.26122 |
| SCOPE | GDM | 19.96015 | 3.6307532 | Inf | 13.974303 | 28.51001 |
| STOP | GDM | 17.43694 | 1.3414818 | Inf | 14.996317 | 20.27478 |
| Study | GDM_new | response | SE | df | asymp.LCL | asymp.UCL |
|---|---|---|---|---|---|---|
| SCOPE | Uncomplicated | 3.426268 | 0.2408458 | Inf | 2.985294 | 3.932382 |
| STOP | Uncomplicated | 2.163895 | 0.0941183 | Inf | 1.987070 | 2.356454 |
| SCOPE | GDM | 4.807918 | 0.9882138 | Inf | 3.213676 | 7.193032 |
| STOP | GDM | 3.750223 | 0.3083980 | Inf | 3.191970 | 4.406109 |
| Study | GDM_new | response | SE | df | asymp.LCL | asymp.UCL |
|---|---|---|---|---|---|---|
| SCOPE | Uncomplicated | 5.447121 | 0.0654643 | Inf | 5.320313 | 5.576952 |
| STOP | Uncomplicated | 4.701641 | 0.0394811 | Inf | 4.624892 | 4.779663 |
| SCOPE | GDM | 5.340856 | 0.2627192 | Inf | 4.849979 | 5.881416 |
| STOP | GDM | 4.850957 | 0.0626863 | Inf | 4.729637 | 4.975390 |
59.7.4 Comparisons
59.7.4.1 Within Groups
Effect for every 1 unit increase in Homocysteine concentration (μM).| Hormone | Contrast | Study | GDM_new | Ratio of Geometric means (95% CI) | P-value |
|---|---|---|---|---|---|
| Prolactin | Homocysteine5.5 / Homocysteine4.5 | SCOPE | Uncomplicated | 0.978 (0.930, 1.027) | 0.4 |
| Prolactin | Homocysteine5.5 / Homocysteine4.5 | STOP | Uncomplicated | 0.967 (0.927, 1.009) | 0.1 |
| Prolactin | Homocysteine5.5 / Homocysteine4.5 | SCOPE | GDM | 0.952 (0.860, 1.053) | 0.3 |
| Prolactin | Homocysteine5.5 / Homocysteine4.5 | STOP | GDM | 0.941 (0.865, 1.024) | 0.2 |
| HPL | Homocysteine5.5 / Homocysteine4.5 | SCOPE | Uncomplicated | 0.990 (0.964, 1.017) | 0.5 |
| HPL | Homocysteine5.5 / Homocysteine4.5 | STOP | Uncomplicated | 0.983 (0.961, 1.006) | 0.1 |
| HPL | Homocysteine5.5 / Homocysteine4.5 | SCOPE | GDM | 0.927 (0.878, 0.979) | 0.006 |
| HPL | Homocysteine5.5 / Homocysteine4.5 | STOP | GDM | 0.921 (0.879, 0.964) | 0.0004 |
| GH2 | Homocysteine5.5 / Homocysteine4.5 | SCOPE | Uncomplicated | 0.980 (0.946, 1.016) | 0.3 |
| GH2 | Homocysteine5.5 / Homocysteine4.5 | STOP | Uncomplicated | 1.003 (0.973, 1.035) | 0.8 |
| GH2 | Homocysteine5.5 / Homocysteine4.5 | SCOPE | GDM | 0.934 (0.868, 1.006) | 0.07 |
| GH2 | Homocysteine5.5 / Homocysteine4.5 | STOP | GDM | 0.956 (0.899, 1.017) | 0.2 |
| Insulin | Homocysteine5.5 / Homocysteine4.5 | SCOPE | Uncomplicated | 0.979 (0.905, 1.059) | 0.6 |
| Insulin | Homocysteine5.5 / Homocysteine4.5 | STOP | Uncomplicated | 0.980 (0.926, 1.038) | 0.5 |
| Insulin | Homocysteine5.5 / Homocysteine4.5 | SCOPE | GDM | 0.576 (0.442, 0.750) | <0.0001 |
| Insulin | Homocysteine5.5 / Homocysteine4.5 | STOP | GDM | 0.870 (0.747, 1.014) | 0.07 |
| HOMA.IR | Homocysteine5.5 / Homocysteine4.5 | SCOPE | Uncomplicated | 0.980 (0.901, 1.067) | 0.6 |
| HOMA.IR | Homocysteine5.5 / Homocysteine4.5 | STOP | Uncomplicated | 0.982 (0.924, 1.044) | 0.6 |
| HOMA.IR | Homocysteine5.5 / Homocysteine4.5 | SCOPE | GDM | 0.518 (0.387, 0.695) | <0.0001 |
| HOMA.IR | Homocysteine5.5 / Homocysteine4.5 | STOP | GDM | 0.865 (0.736, 1.017) | 0.08 |
| GLUC3_mmol.L | Homocysteine5.5 / Homocysteine4.5 | SCOPE | Uncomplicated | 1.001 (0.987, 1.015) | 0.9 |
| GLUC3_mmol.L | Homocysteine5.5 / Homocysteine4.5 | STOP | Uncomplicated | 0.995 (0.980, 1.010) | 0.5 |
| GLUC3_mmol.L | Homocysteine5.5 / Homocysteine4.5 | SCOPE | GDM | 0.870 (0.790, 0.958) | 0.005 |
| GLUC3_mmol.L | Homocysteine5.5 / Homocysteine4.5 | STOP | GDM | 0.993 (0.971, 1.016) | 0.5 |
59.7.4.2 Between Groups
Below are comparisons of the differences in the change in Homocysteine concentration (μM) between groups. I.e. whether the change in Homocysteine concentration (μM) are different between groups.
| contrast | ratio | SE | df | lower.CL | upper.CL | p.value | |
|---|---|---|---|---|---|---|---|
| SCOPE/SCOPE (Overall) | 0.9787372 | 0.0640855 | 1417.025 | 0.8607632 | 1.112880 | 0.743 | ns |
| STOP/SCOPE (Uncomplicated) | 0.9893115 | 0.0323889 | 1417.025 | 0.9277733 | 1.054931 | 0.743 | ns |
| STOP/SCOPE (GDM) | 0.9893115 | 0.0323889 | 1417.025 | 0.9277733 | 1.054931 | 0.743 | ns |
| GDM/Uncomplicated (SCOPE) | 0.9732322 | 0.0452715 | 1412.965 | 0.8883569 | 1.066217 | 0.560 | ns |
| GDM/Uncomplicated (STOP) | 0.9732322 | 0.0452715 | 1412.965 | 0.8883569 | 1.066217 | 0.560 | ns |
| contrast | ratio | SE | df | lower.CL | upper.CL | p.value | |
|---|---|---|---|---|---|---|---|
| SCOPE/SCOPE (Overall) | 0.9861437 | 0.0348326 | 1400.365 | 0.9201276 | 1.0568962 | 0.693 | ns |
| STOP/SCOPE (Uncomplicated) | 0.9930477 | 0.0175382 | 1400.365 | 0.9592328 | 1.0280546 | 0.693 | ns |
| STOP/SCOPE (GDM) | 0.9930477 | 0.0175382 | 1400.365 | 0.9592328 | 1.0280546 | 0.693 | ns |
| GDM/Uncomplicated (SCOPE) | 0.9366159 | 0.0236704 | 1397.986 | 0.8913147 | 0.9842196 | 0.010 | ** |
| GDM/Uncomplicated (STOP) | 0.9366159 | 0.0236704 | 1397.986 | 0.8913147 | 0.9842196 | 0.010 | ** |
| contrast | ratio | SE | df | lower.CL | upper.CL | p.value | |
|---|---|---|---|---|---|---|---|
| SCOPE/SCOPE (Overall) | 1.0475947 | 0.0496954 | 1468.841 | 0.9545111 | 1.149756 | 0.327 | ns |
| STOP/SCOPE (Uncomplicated) | 1.0235207 | 0.0242767 | 1468.841 | 0.9769909 | 1.072267 | 0.327 | ns |
| STOP/SCOPE (GDM) | 1.0235207 | 0.0242767 | 1468.841 | 0.9769909 | 1.072267 | 0.327 | ns |
| GDM/Uncomplicated (SCOPE) | 0.9532964 | 0.0323553 | 1427.051 | 0.8918941 | 1.018926 | 0.159 | ns |
| GDM/Uncomplicated (STOP) | 0.9532964 | 0.0323553 | 1427.051 | 0.8918941 | 1.018926 | 0.159 | ns |
| contrast | ratio | SE | df | asymp.LCL | asymp.UCL | p.value | |
|---|---|---|---|---|---|---|---|
| SCOPE/SCOPE (Overall) | 1.5134372 | 0.2464932 | Inf | 1.0998398 | 2.082569 | 0.011 | * |
| STOP/SCOPE (Uncomplicated) | 1.0011112 | 0.0496046 | Inf | 0.9084598 | 1.103212 | 0.982 | ns |
| STOP/SCOPE (GDM) | 1.5117573 | 0.2347271 | Inf | 1.1151106 | 2.049492 | 0.008 | ** |
| GDM/Uncomplicated (SCOPE) | 0.5879472 | 0.0826295 | Inf | 0.4463867 | 0.774400 | 0.0002 | *** |
| GDM/Uncomplicated (STOP) | 0.8878469 | 0.0734313 | Inf | 0.7549838 | 1.044091 | 0.150 | ns |
| contrast | ratio | SE | df | asymp.LCL | asymp.UCL | p.value | |
|---|---|---|---|---|---|---|---|
| SCOPE/SCOPE (Overall) | 1.6721775 | 0.2986039 | Inf | 1.1783700 | 2.3729199 | 0.004 | ** |
| STOP/SCOPE (Uncomplicated) | 1.0017903 | 0.0535375 | Inf | 0.9021672 | 1.1124143 | 0.973 | ns |
| STOP/SCOPE (GDM) | 1.6691892 | 0.2844275 | Inf | 1.1952591 | 2.3310367 | 0.003 | ** |
| GDM/Uncomplicated (SCOPE) | 0.5288124 | 0.0822546 | Inf | 0.3898528 | 0.7173029 | <0.0001 | *** |
| GDM/Uncomplicated (STOP) | 0.8811105 | 0.0772880 | Inf | 0.7419351 | 1.0463931 | 0.149 | ns |
| contrast | ratio | SE | df | asymp.LCL | asymp.UCL | p.value | |
|---|---|---|---|---|---|---|---|
| SCOPE/SCOPE (Overall) | 1.1337832 | 0.0589925 | Inf | 1.0238604 | 1.2555076 | 0.016 | * |
| STOP/SCOPE (Uncomplicated) | 0.9938672 | 0.0106101 | Inf | 0.9732879 | 1.0148816 | 0.564 | ns |
| STOP/SCOPE (GDM) | 1.1407794 | 0.0578375 | Inf | 1.0328702 | 1.2599624 | 0.009 | ** |
| GDM/Uncomplicated (SCOPE) | 0.8696156 | 0.0432179 | Inf | 0.7889047 | 0.9585839 | 0.005 | ** |
| GDM/Uncomplicated (STOP) | 0.9981612 | 0.0136557 | Inf | 0.9717522 | 1.0252879 | 0.893 | ns |